JATROMA project

Jatropha curcas

Jatropha curcas L. (Euphorbiaceae) is one of the most promising plant for biodiesel production.
Today’s varieties show suboptimal quantitative and qualitative traits, such as low yield and oil content and the presence of toxic and anti-nutritional compounds, while its genetic variability and economic yield potentials remain unexploited.
With the JATROMA Project Bioma Technologies is developing enhanced jatropha elite varieties through the establishment of a germplasm core collection, its genetic and biochemical characterization and the development of breeding programs.

Jatropha curcas

    Germplasm core collection:
  • Our germplasm core collection consists of several hundreds accessions collected around the centre of origin of the species (Central America). Each accession is represented by seeds collected from wild plants for which geographical location was recorded. Genetic and biochemical analyses has revealed a very high genetic variability of the collection and the presence of several non-toxic genotypes that can be implemented into breeding programs.

    Genetic marker analysis:
  • Bioma Technologies has developed proprietary single sequence repeats (SSR) molecular markers liked to jatropha traits of interest for the characterization of its germplasm core collection. By a focused resequencing project of the most variable accessions Bioma Technologies has developed a novel SNP-panel assay able to characterize each Jatropha curcas chromosome; this panel allows for accurate genetic distance analysis and trait-specific selections.

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    Poster: Genetic characterization of Jatropha curcas L. accessions from different countries


    Biochemical analysis:
  • Bioma Technologies has developed High Performance Liquid Chromatography (HPLC)-based protocols for the analysis of toxic compounds, such as phorbol esters, curcin and other secondary metabolites from jatropha seeds, panel and leaves.

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    Jatropha micropropagation:
  • Bioma Technologies has develop proprietary in-vitro embryogenesis and organogenesis protocols in jatropha for massive propagation of individual accession. Our combined in-vitro and greenhouse facilities allows for the high-throughput multiplication of interesting genotypes in a short period of time.

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    Poster: Plant cell cultures from Jatropha curcas
    Poster: In vitro cell cultures from Jatropha curcas